MIP-Based Sensor for Detection of Gut Microbiota Derived Trimethylamine

Abstract

Trimethylamine (TMA), a gut microbiota-based metabolite is linked to human health and illness. The detection of TMA in bodily fluids is believed to be crucial in extrapolating the pathophysiology and treatment of a variety of disorders. As a result, the development of accurate and fast TMA detection methods might help us better understand the pathophysiology and diagnosis of disorders linked to TMA. The current study is the first to describe the construction of an electrochemical sensor based on a molecularly imprinted polymer (MIP) for specific and sensitive detection of TMA in human body fluids such as urine. The MIP design was based on polydopamine (PDA), which was made with and without TMA using a polymerization technique. On the glass coated with indium tin oxide (ITO), the MIP, NIP, and non-sonicated polymer (PPy-TMA) were individually deposited. Differential pulse voltammetry (DPV) was used to record the detection response and obtained a sensitivity of 80 nA ml μM−1 within a dynamic detection range of 0.5 μM to 400 mM. This sensor is simple to build; use and it can detect TMA in human fluids like urine with great precision. The current study lays the groundwork for developing MIP-based sensors to detect additional compounds that play role in human health and are originating from gut microbiota.