Abstract
To find out whether macrophage inflammatory protein-1α (MIP-1α) has a role in the regulation of germ cell development, we studied its effects on spermatogenic stage-specific DNA synthesis in vitro. MIP-1α increased the DNA synthesis of primitive type A 2–4 spermatogonia and of premeiotic cells, whereas the DNA synthesis of more differentiated intermediate and type B spermatogonia was inhibited when cultured in the presence of MIP-1α. An antibody against MIP-1α cross-reacted with a protein of 15 kDa from every spermatogenic stage of rat seminiferous epithelium. Immunohistochemical studies with the same antibody revealed a complex pattern of MIP-1α localization both in primitive and advanced spermatogenic cells. These observations suggest that MIP-1α is a local regulator of mitotic and meiotic DNA synthesis.
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