Abstract
The teleost Poecilia vivipara are characterized as euryhaline, in other words, they modulate gill cell behavior to survive in environments with varying salinity. P.vivipara gills have a set of striated skeletal muscles, abductor and adductor muscles, which plays an important role in the respiration process. This study aimed to describe the morphology of gill muscles during the early stages of development and perform an initial morphological characterization of adults P.vivipara. Moreover, it aimed to analyze the effects of different concentrations of salinity on the morphological characteristics of muscle fibers in fingerlings´ gills. Results showed that the muscle fibers are formed between phases 2 and 3 of embryonic development. At this stage of development, gill muscles are inserted into the basal portion of gill filament, possibly in the bulkhead of cartilage that supports the gills, and organized in muscle bundles located along the gill filament. This morphological organization remains in adults. An increase in the diameter of the fibers and muscle bundles was observed in fingerlings exposed to concentrations of sea salt, which allows relating the body size of P.vivipara with the degree of salinity of the environment where they live. KEYWORDS: guppy; gill muscle; ontogeny; salinity.
Highlights
The teleost Poecilia vivipara are characterized as Results showed that the muscle fibers are formed between euryhaline, in other words, they modulate gill cell phases 2 and 3 of embryonic development
Essa musculatura dos filamentos branquiais desempenha a ação de bombear a água através das brânquias, atuando no direcionamento do fluxo da água, tendo então papel nos mecanismos de respiração (BALLINTIJN et al, 1985)
Dessa forma, supõe-se que os alevinos de Poecilia vivipara expostos à salinidade aumentaram o diâmetro das fibras e feixes musculares devido a mecanismos de osmorregulação
Summary
Os espécimes de Poecilia vivipara foram coletados em tanques do setor de piscicultura da Escola de Medicina Veterinaria e Zootecnia da Universidade Federal de Goiás, onde os tanques correspondiam às águas continentais habituais da espécie. Os animais foram aclimatados por um período de 48 horas em tanque com 40L de água doce (AD). No primeiro grupo 15 fêmeas foram sacrificadas por decapitação e do seu ventre coletados embriões em diferentes estágios de desenvolvimento, sendo o estágio de cada espécime avaliado através de microscopia estereoscópica. Além da coleta dos embriões, foram coletados deste grupo de fêmeas os arcos branquiais, para a análise das brânquias de espécimes adultos. Os alevinos foram retirados da água doce (AD) e expostos à água com diferentes salinidade (0‰, 5‰, 10‰, 15‰ e 20‰) (sal marinho comercial Coralife - USA), em sistema estático sem renovação da água, por um período total de 2 horas. Os arcos branquiais oriundos dos espécimes adultos foram inclusos em paraplast, seccionados a 4μm de espessura e corados com Hematoxilina-Eosina.
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