Abstract
Publisher Summary This chapter summarizes the studies directed toward characterizing the cis -acting sequences that are required for minute virus of mice (MVM) genome replication and discusses the biochemical properties of NS-1, the major nonstructural protein of MVM, and the way it is involved in the replication of the genome. The modified rolling hairpin model for MVM replication was proposed some years ago. This model introduced the idea that the resolution of the central bridge dimer involves an asymmetric procedure that could explain the way a unique sequence is retained at the left-hand end of the MVM genome. To assay replication competence of molecular clones of the MVM genome, plasmids containing the MVM DNA and a source of NS-1 are transfected into permissive mouse fibroblast cells or nonpermissive simian COS-7 cells.
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