Minor Physiological Response to Elevated CO2 by the CAM Plant Agave vilmoriniana

Abstract

One-year-old plants of the CAM leaf succulent Agave vilmoriniana Berger were grown outdoors at Riverside, California. Potted plants were acclimated to CO(2)-enrichment (about 750 microliters per liter) by growth for 2 weeks in an open-top polyethylene chamber. Control plants were grown nearby where the ambient CO(2) concentration was about 370 microliters per liter. When the plants were well watered, CO(2)-induced differences in stomatal conductances and CO(2) assimilation rates over the entire 24-hour period were not large. There was a large nocturnal acidification in both CO(2) treatments and insignificant differences in leaf chlorophyll content. Well watered plants maintained water potentials of -0.3 to -0.4 megapascals. When other plants were allowed to dry to water potentials of -1.2 to -1.7 megapascals, stomatal conductances and CO(2) uptake rates were reduced in magnitude, with the biggest difference in Phase IV photosynthesis. The minor nocturnal response to CO(2) by this species is interpreted to indicate saturated, or nearly saturated, phosphoenolpyruvate carboxylase activity at current atmospheric CO(2) concentrations. CO(2)-enhanced diurnal activity of ribulose bisphosphate carboxylase activity remains a possibility.