Minor Actinides Can Replace Essential Lanthanides in Bacterial Life.

Abstract

Over the last decade, the biological relevance of certain f-elements - the lanthanides - was established in the context of methylotrophic bacteria. The respective strains incorporate these 4f-elements into the active site of one of their key metabolic enzymes, a lanthanide-dependent methanol dehydrogenase. Here, we investigate if actinides, the radioactive 5f-elements, can replace the essential 4f-elements in lanthanide-dependent bacterial metabolism. Growth studies with Methylacidiphilum fumariolicum SolV and the Methylobacterium extorquens AM1 ΔmxaF-mutant demonstrate that americium and curium support growth in the absence of lanthanides. Moreover, strain SolV favors these actinides over late lanthanides, when presented to a mixture of equal amounts of lanthanides together with americium and curium. Bacterial uptake is confirmed by time-resolved laser-induced fluorescence spectroscopy, liquid scintillation counting, and inductively-coupled plasma mass-spectrometry. Additionally, we show catalytic activity of the lanthanide-dependent enzyme upon reconstitution with actinides. Our combined invivo and in vitro results establish that methylotrophic bacteria can utilize actinides instead of lanthanides to sustain their one-carbon metabolism; if they possess the correct size and a +III oxidation state. Furthermore, bacteria capable of utilizing actinides will be able to mobilize these elements in the environment. This may lead to applications for bioremediation and recycling/separation of lanthanides and actinides.