Abstract
Minimum fungicidal concentrations (MFCs) of amphotericin B were obtained for 165 bloodstream isolates (104 Candida parapsilosis, 14 C.glabrata, 13 C.tropicalis, 15 C.krusei, and 19 C.albicans) and 36 C.dubliniensis from oropharyngeal infections. Minimum inhibitory concentrations (MICs) were determined by the M27-A microdilution method. MFCs (≥99.9% killing) were obtained following MIC determination (inoculum size, 10 4 CFU/ml) by seeding the entire volume of all clear wells. The best fungicidal activity was for C. albicans, (MFC 90 1 μg/ml) and the lowest for C.parapsilosis, C.tropicalis and C.glabrata (MFC 90 16 μg/ml). Although MFCs were ≥16× MIC for some isolates, including C. glabrata, the overall MFCs were ≥2× MICs. However, major differences between MICs and MFCs were observed for C.parapsilosis and C.dubliniensis (3.8% and 8.9%, respectively, were tolerant: MFC ≥32MIC). MFCs for C.tropicalis and C. glabrata were ≥2 μg/ml. By this more stringent method we found substantial differences from those previously reported between amphotericin B MIC and MFCs for Candida spp.
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