Abstract
The volumetric oxygen transfer coefficient (kLa) was used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation in bioleaching cultures ofAcidithiobacillus ferrooxidans. The Michaelis constants for oxygen were 1.07 and 0.71 μmol O2l-1for the oxidation of ferrous iron and elemental sulphur, respectively. The critical oxygen concentration, below which oxygen limitation occurred, was determined to be 6.25 and 3.125 μmol O2l-1for the oxidation of ferrous iron and elemental sulphur, respectively. The (kLa)critvalues required to maintain oxygen-unlimited substrate oxidation for ferrous iron and elemental sulphur were 7.70 and 4.88 h-1, respectively.
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