Abstract

Excessive post-washing of pretreated biomass leads to huge water consumption and chemical loss. To address this issue, parallel HOAc and NaOH pretreatments of biomass followed by integration of their biomass and filtrate were investigated. Pretreatment effectiveness including morphology, crystallinity, and component recovery, were elucidated. Results showed that HOAc and NaOH in the mixed filtrate were neutralized to achieve a pH of around 4.80 prompting the alkali lignin precipitation. Lignin (46.01 and 48.38 g/kg-biomass for hemp and poplar, respectively) exhibiting comparable FTIR characteristics with the commercial alkali lignin was recovered. Compared to sodium acetate buffer as a control, integrating HOAc and NaOH pretreated biomass and their mixed filtrate for enzymatic hydrolysis boosted total sugar concentration (hemp: 42.90 vs. 38.27 g/L; poplar: 43.18 vs. 38.76 g/L) without compromising glucose yield (hemp: 70.86 vs. 70.69%; poplar: 66.48 vs. 69.48%) but improving xylose yield (hemp: 60.10 vs. 35.92%; poplar: 56.90 vs. 29.39%).

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