Abstract
Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. The present study purposed to describe a minimally invasive surgical protocol for adipose tissue collection from sheep�s inter-scapular area. The procedure was carried out on adult sheeps, in aseptic conditions. A light sedation protocol with Detomidine was performed, the recovery from anesthesia being carried out with Atipamezole. Throughout the sedation, the surgical procedure and the recovery from anesthesia, the vital functions of the animal were monitored. The adipose tissue samples collected in sterile tubes with culture medium (Dulbecco�s modified Eagle�s medium - DMEM/10% / FBS10 - fetal bovine serum, 2% antibiotic/antifungal), have been succesfully used by our research team for adipose tissue derived stem cells (ADSCs) isolation for further use in cardiac valves tissue engineering.
Highlights
Many methods of stem cells isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering
This study aimed to present a minimally invasive surgical protocol used by our research team for adipose tissue collection and isolation of adipose tissue derived stem cells (ADSCs) in cardiac valves tissue regeneration
Vomiting, muscle twitching or hypothermia were not registered as well after recovery from sedation. tAahthiepigarhmesvepezeorcsleiaf,ilcainotyfαfos2 reatddhraeetαnive2e-ragdaicrnerndeecraegnpicatolrgreecaesniptcatogerosfn,fieassct,stuwsrieothdf Detomidine, waking up the animals within 10 min. It was not registered any case of postoperative wound infection or dehiscence, all experimental animals recovered quickly and favorably without any type of complication
Summary
Many methods of stem cells collection and isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. Many methods of stem cells isolation from various tissue types harvested either from small or large experimental animals or from human tissues have been published so far, all evaluating them as a potential source of adult mesenchymal stem cells with applicability in various pathologies or tissue bioengineering. Due to the easy protocol of collection, isolation and the great potential of proliferation and tissue differentiation in multiple cell lines, adipose tissue proved to be an attractive source of multipotent stem cells with potential use in regenerative medicine and tissue engineering [14].
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