Abstract
We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excised unstained animal vascular tissues.
Highlights
DEUTSCHE PHYSIKALISCHE GESELLSCHAFT between the lifetimes and initial intensities [7]
This study aims (i) to develop a fluorescence lifetime imaging microscopy (FLIM) apparatus using a side-viewing flexible fibre bundle probe that may be made compatible with intravascular catheters and flexible endoscopic probes; (ii) to apply the more robust and computationally fast Laguerre polynomial approach [5] to fitting FLIM data collected with the flexible probe, and (iii) to determine whether the spectral lifetime signature of tissues specimens that have been previously observed with single point spectroscopy can be retrieved from FLIM data
We have demonstrated an instrument for fluorescence lifetime imaging in a flexible sideviewing endoscope that is compatible with intravascular imaging constraints
Summary
When only a few, fixed emission wavelength bands are of interest, a dichroic based image splitting device is an attractive option In this case multiple spectrally resolved images of the sample are arranged on a single detector and image registration methods are used to extract the spectral information pixel by pixel [8]. Single point endoscopic probes for recording lifetime and/or emission resolved spectroscopy are being widely applied [3, 10], there have been relatively few reports of FLIM applied through endoscope systems.A frequency-domain endoscopic FLIM instrument for videorate FLIM of biological tissue was reported with a spatial resolution of 32 × 32 pixels, recording phase resolved images and assuming a mono-exponential decay [11].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
