Abstract
A rapid and simple method for the preparation of a miniature glucose sensor (enzyme electrode) has been developed. Glucose oxidase is immobilized by glutaraldehyde in albumin gel on a cellulose diacetate membrane. This membrane is directly attached to an H 2O 2 detecting electrode and covered with an external polyvinyl chloride membrane. The response time of the glucose electrode thus prepared is about 2 min, the calibration graph is linear for 0–30 mM glucose, and the sensor can be used repeatedly at room temperature for at least 10 days with small deterioration in response.
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