Abstract
Microfluidics is becoming a technology of growing interest to build miniature culturing systems, capable of mimicking tissue functions and multi-tissue interactions in so-called “body-on-a-chip ” applications while featuring integrated readout functionalities. This chapter presents a highly versatile, modular and scalable analytical platform technology, which combines microfluidic hanging-drop networks with multi-analyte biosensors for in situ monitoring of the metabolism of 3D microtissues. The microfluidic platform is based on the hanging -drop network technology, which has been designed for formation, cultivation, and analysis of fluidically interconnected organotypic spherical 3D microtissues that can be obtained from various different cell types. The sensor modules were designed as small glass plug-ins, which allow for convenient functionalization and calibration of the sensors and do not interfere with the microfluidic functions. They were placed in the ceiling substrate, from which the hanging drops that host the spheroid cultures were suspended. The detection of secreted lactate of single microtissue spheroids will be presented. Further, we will demonstrate that it is possible to monitor microtissue lactate secretion and glucose consumption in parallel.
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