Mini-Review: Transgenerational CRISPR/Cas9 Gene Editing in Plants.

Lennert Impens,Laurens Pauwels,Thomas B Jacobs,Hilde Nelissen,Dirk Inzé

doi:10.3389/fgeed.2022.825042

Lennert Impens, Laurens Pauwels + Show 3 more

Open Access

https://doi.org/10.3389/fgeed.2022.825042

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Abstract

CRISPR/Cas9 genome editing has been used extensively in a wide variety of plant species. Creation of loss-of-function alleles, promoter variants and mutant collections are a few of the many uses of genome editing. In a typical workflow for sexually reproducing species, plants are generated that contain an integrated CRISPR/Cas9 transgene. After editing of the gene of interest, T-DNA null segregants can be identified in the next generation that contain only the desired edit. However, maintained presence of the CRISPR/Cas9 transgene and continued editing in the subsequent generations offer a range of applications for model plants and crops. In this review, we define transgenerational gene editing (TGE) as the continued editing of CRISPR/Cas9 after a genetic cross. We discuss the concept of TGE, summarize the current main applications, and highlight special cases to illustrate the importance of TGE for plant genome editing research and breeding.

Highlights

CRISPR/Cas9 has rapidly become the predominant tool for plant genome editing (Chen et al, 2019)
CRISPR/Cas9 Editing in Arabidopsis Using Floral Dip In CRISPR/Cas9 editing of Arabidopsis, T1 genotypes can be viewed as the result of transgenerational gene editing (TGE) as a novel WT allele is introduced after transformation of the haploid female gametophyte
CRISPR/Cas9-based genome editing already is an indispensable tool in plant genetics and breeding and many new technologies are being developed to expand the CRISPR toolbox such as base and prime editing

Summary

Introduction

CRISPR/Cas9 has rapidly become the predominant tool for plant genome editing (Chen et al, 2019). Researchers typically use CRISPR/Cas9 to target exonic open reading frames to generate loss-offunction mutants for functional analysis Somatic mosaic mutational patterns may indicate that the CRISPR/Cas9 machinery is not always active immediately after Agrobacterium-mediated delivery and that different cell lineages already were established.

Results

Conclusion