Abstract
The degradation of uniformly 14 C-labeled isomers (α, γ, and β) of hexachlorocyclohexane (HCH), added separately to a mineral salts medium as a sole source of carbon, by a soil bacterium, Pseudomonas sp., under aerobic conditions was studied. During aerobic degradation, about 10-12% of the 14 C in α- and γ-HCH was accounted for as 14 CO 2 as compared to 5% from β-HCH. Most of the 14 C in α- and γ-HCH accumulated as water-soluble products, while formation of water-soluble products from β-HCH was negligible. Almost all of the 14 C in the three isomers of HCH was accounted for in different fractions (CO 2 , chloroform-diethyl ether, water phase) following bacterial degradation under aerobic conditions. GC-MS analysis of β-HCH residues in the chloroformdiethyl ether extract suggested the formation of pentachlorocyclohexanol and tetrachlorocyclohexanediol as products of aerobic metabolism of β-HCH
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