Mineral-Associated Soil Carbon is Resistant to Drought but Sensitive to Legumes and Microbial Biomass in an Australian Grassland

Alberto Canarini,Pierre Mariotte,Andrew Merchant,Feike A Dijkstra,Lachlan Ingram

doi:10.1007/s10021-017-0152-x

Abstract

Drought is predicted to increase in many areas of the world with consequences for soil carbon (C) dynamics. Plant litter, root exudates and microbial biomass can be used as C substrates to form organo-mineral complexes. Drought effects on plants and microbes could potentially compromise these relative stable soil C pools, by reducing plant C inputs and/or microbial activity. We conducted a 2-year drought experiment using rainout shelters in a semi-natural grassland. We measured aboveground biomass and C and nitrogen (N) in particulate organic matter (Pom), the organo-mineral fraction (Omin), and microbial biomass within the first 15 cm of soil. Aboveground plant biomass was reduced by 50% under drought in both years, but only the dominant C4 grasses were significantly affected. Soil C pools were not affected by drought, but were significantly higher in the relatively wet second year compared to the first year. Omin-C was positively related to microbial C during the first year, and positively related to clay and silt content in the second year. Increases in Omin-C in the second year were explained by increases in legume biomass and its effect on Pom-N and microbial biomass N (MBN) through structural equation modeling. In conclusion, soil C pools were unaffected by the drought treatment. Drought resistant legumes enhanced formation of organo-mineral complexes through increasing Pom-N and MBN. Our findings also indicate the importance of microbes for the formation of Omin-C as long as soil minerals have not reached their maximum capacity to bind with C (that is, saturation).

Highlights

Grassland ecosystems represent between 30 and 40% of the global land surface area, storing organic C in amounts comparable to forest ecosystems (White and others 2000)
Time was almost always significant, causing an increase in microbial biomass N (MBN), NH4+, organomineral fraction (Omin)-C and particulate organic matter (Pom)-C, and a decrease in dissolved organic nitrogen (DON) in January 2016 compared to January 2015
microbial biomass carbon (MBC) was positively related with Organo-mineral C (Omin-C) in January 2015, but this relationship was lost in January 2016 (Figure 3A)

Summary

Introduction

Grassland ecosystems represent between 30 and 40% of the global land surface area, storing organic C in amounts comparable to forest ecosystems (White and others 2000). Soil organic matter (SOM) decomposition (that is, C outputs from soil) is often maintained (Hoover and Rogers 2016), the response of SOM decomposition to drought will depend on drought intensity and timing of rewetting periods (Bloor and Bardgett 2012). Because SOM is made of C pools with different inherent levels of turnover and stability (Six and others 2002), understanding the C dynamics of different pools in response to environmental stress is critical to assess impacts of grassland ecosystems on CO2 release to the atmosphere. Understanding drought effects on C pools is important for predicting climate change feedbacks in grassland ecosystems and possible legacy effects of post-drought periods

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