Abstract

White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). This study aimed to investigate the effects of NaOCl on the surface properties of WMTA discs and WMTA-induced osteoblastic differentiation of periodontal ligament stem cells (PDLSCs). Mixed WMTA (ProRoot MTA) was filled into the molds to form WMTA discs. These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA). Their surface structures and Ca2+ release level was investigated. Moreover, they were cultured with a clonal human PDLSC line (line 1–17 cells). The main crystal structures of Na-WMTA were identical to the structures of D-WMTA. Globular aggregates with polygonal and needle-like crystals were found on D-WMTA and Na-WMTA, which included Ca, Si, Al, C and O. However, many amorphous structures were also identified on Na-WMTA. These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion also reduced Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. However, cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes. These results indicated that NaOCl reduced Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. NaOCl may also partially abolish the ability of WMTA to stimulate osteoblastic differentiation of PDLSCs.

Highlights

  • White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl)

  • Crystalline structures of WMTA discs without immersion (ContWMTA), D-WMTA, and Na-WMTA were investigated by X‐ray diffraction (XRD) (D8-Advance A25, Bruker, Karlsruhe, Germany) using CuKα radiation operated at 40 kV and 40 mA

  • NaOCl induced discoloration of WMTA discs at concentrations from 2 to 0.01%; WMTA discs in contact with 0.001% and 0.0001% NaOCl exhibited a color similar to discs immersed in distilled water (DW) (Supplementary Fig. 1)

Read more

Summary

Introduction

White mineral trioxide aggregate (WMTA) is a root canal treatment material, which is known to exhibit a dark brown color when in contact with sodium hypochlorite solution (NaOCl). These discs were immersed in distilled water (D-WMTA) or 5% NaOCl (Na-WMTA) Their surface structures and ­Ca2+ release level was investigated. Many amorphous structures were identified on Na-WMTA These structures consisted of Na and Cl, but did not include Ca. NaOCl immersion reduced ­Ca2+ release level from whole WMTA discs. Line 1–17 cells cultured with D-WMTA formed many mineralized nodules and exhibited high expression levels of osteoblast-related genes. Cells incubated with Na-WMTA generated a small number of nodules and showed low expression levels of osteoblast-related genes These results indicated that NaOCl reduced ­Ca2+ release from WMTA by generating amorphous structures and changing its elemental distribution. Several reports have described its effects on WMTA; contact between WMTA and NaOCl results in dark brown discoloration of ­WMTA7, as well as reduced formation of calcium hydroxide (Ca [­ OH]2)[8]

Objectives
Methods
Results
Conclusion

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.