Abstract
A simple and effective method for the substitution of mineral oil for xylene in tissue processing is described. After a short dehydration with ethanol, tissue fragments are immersed in a mixture of ethanol, isopropyl alcohol, and mineral oil at 45°C and 50°C, with vacuum / pressure /agitation in order to complete the dehydration and initiate the infiltration that is finalized with mineral oil and melted paraffin wax in separate and consecutive steps.Using random samples from 38 different types of tissue, 92 pairs of slides were prepared, half from specimens processed with xylene and the other half with the new procedure. Each of the 92 pairs of slides, that included 12 different conventional histochemical stains and 21 different immunohistocliemical stains, was evaluated 9 times in a blind test by as many reviewers. The evaluation of the results with the X2 test showed that processing tissue with mineral oil is equivalent to processing tissue with xylene. Furthermore, mineral oil poses no health or environmental risks and has microtomy advantages as well. Since June199 this new procedure Ilas been the standard method for tissue processing at Mount Sinai Meclical Center of Greater Miami. (The J Histotechnol 23:143, 2000)
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