Mimotope analysis of helicobacter pylori catalase based on mimox tool

Abstract

Objective To analyze the characteristics of mimotopes from Helicobacter pylori Catalase using Mimox software and compare with manually alignment.Methods Five mimotopes derived from phage display library were input Mimox web (http://immunet.cn/mimox/) and aligned with all parameters as defaults.Results The interlace ClustalW aligned the set of mimotopes and the appearance frequency of amino acids P,T,S,L,A was higher.The statistical method deduced the consensus sequence-[ HST] X [DST] FRPXA [ TNQ ] [ LV ] [ FW ] T-,in which the appearance frequency of amino acids P ( 80％ ) and A (60％) was higher.The interface JalView deduced the consensus sequence-H-DFRP-AT-T-,in which the conservation of amino acid T( value 8),P( value 6),D( value 5 ) was higher,the quality of amino acid T( value 2.4595077),P( value 2.235784),R(value 2.1615076) was higher,the consensus of amino acid P( 80％ ),A(60％ ),T(60％ )was higher.The results were consistent with manul alignment although there exited discrepancy.Conclusion The combination of phage display library and Mimox may analyze characteristics of minotopes and obtain comprehensive epitope information. Key words: Mimox Software; Mimotope; Helicobacter pylori; Catalase