Abstract
By correlated visual and microscopical examination, milky disease of field-infected, third-instar Japanese beetle larvae is categorized into four phases. The phases are described as sequential disease symptoms I through IV. All four phases persist simultaneously throughout experimental incubation. Larvae die during all phases of the disease; however, the largest percentage of death is at phase II and III of the infectious process. At phase II, 90% of the total population of cell types in the infected hemolymph are vegetative cells; in phase III 65–76% are vegetative cells with 17–28% spores. The massive spore population (95% of population) that characterizes milky disease is designated phase IV; less than 30% of larvae reach this phase of the disease.
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