Abstract
Conventional atmospheric glycerol organosolv pretreatment is energy-intensive with the requirement of long time and/or high temperature. Herein, acid-catalyzed atmospheric glycerol organosolv (ac-AGO) pretreatment was developed under a mild condition to modify the sugarcane bagasse structure for improving enzymatic hydrolyzability. Using single factor and central composite design experiments, ac-AGO pretreatment was optimized at 200 °C for 15 min with 0.06% H2SO4 addition, wherein the hemicellulose and lignin removal rates were 82 and 52%, respectively, with extremely high cellulose retention of 98%. The ac-AGO-pretreated substrate exhibited good enzymatic hydrolyzability at a modest cellulase loading, affording a 70% glucose yield after 72 h. Multiple analysis tools were used to correlate the hydrolyzability of the substrate with its structural features. The results indicated that the mild ac-AGO pretreatment can modify the lignocellulosic biomass structure to achieve good hydrolyzability, mainly resulting in significant hemicellulose removal.
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