Mikrochirurgische zellkerntransplantation bei säugetierzellen

Abstract

It was shown in our experiments that the nuclei from mammalian cells could be transplanted into other mammalian cells. The transplanted nuclei were able to take over typical nuclear function within the recipient cells. This could be proved by the formation of melanosomes following transplantation of nuclei from Harding-Passey melanoma cells into muscle cells. It was possible to demonstrate that the transplanted nuclei could divide in the recipient cells using microscopic and autoradiographic methods and chromosome analysis. In these experiments ascites-tumor cells were used as donors and cultivated mice-embryonic cells as recipients.