Migration mechanism of microcystin-LR synergism with Cu2+ from algal cell to water environment using modified nano system

Abstract

Effects on various macromolecules in algal cells occurred when heavy metals migrated to algal cells from the water environment. An innovative nanosystem was introduced to explore the synergistic process of MC-LR and Cu2+ in algal cells.In this method, the complex groups of Cu2+ and MC-LR were deeply analyzed by infrared spectroscopy, and the migration process and mechanism of MC-LR in cells were explored. The complexation of MC-LR with Cu2+ changed the exposure of hydrophobic and hydrophilic residues and affected their migration from intracellular to aqueous environment. Cu2+ mainly bound to =N-H in guanidine group of MC-LR, which changed the exposure of hydrophobic and hydrophilic residues of MC-LR and affected its migration from intracellular to water environment. In this paper, the effects of Cu2+ concentration and pH on the optimal coordination of MC-LR were studied. When the concentration of Cu2+ was 0.05 mm-2 mm, the complex of Cu2+ and MC-LR had synergistic amplification effect on the detection signal of the nanosystem, and the relative signal value reached the maximum when the pH value was 7. Part of the free copper ions complexed with MC-LR with changing conformation before migrating to the water environment.