Migrated silicon dioxide nanoparticles activates the rice immunity for systemic resistance against two pathogens

Abstract

Rice, the world's primary staple food, is under severe threat from several devastating diseases. To sustainably management rice diseases, developing safe, environmentally friendly alternatives urgently need to be developed. In this study, we synthesized two silicon dioxide nanoparticles, spherical mesoporous silica nanoparticles (MSNs) and virus-like mesoporous silica nanoparticles (VMSNs), and we performed a resistance assay on rice for two major diseases, bacterial blight caused by Xanthomonas oryzae pv. oryzae and sheath blight caused by Rhizoctonia solani. Compared to the control, the two nanoparticle treatments increased rice resistance, with VMSNs exhibiting the highest efficacy in controlling these two diseases, causing a shorter lesion length than those plants treated by MSNs. Coincidentally, the foliar application of VMSNs activates a higher expression level of several pathogenesis-related (PR) genes compared to those of MSNs and SiO2 treatment. By using fluorescein isothiocyanate (FITC)-labeled VMSNs (VMSNs-FITC) to soak the top expanded leaf tips or roots, we observed that the fluorescence of the nanoparticles firstly accumulated at the local site of the top leaves or roots, rapidly migrated to the hypocotyl of rice, and then redistributed sequentially from the bottom leaves to the upper leaves. Furthermore, the foliar or root application of VMSNs-FITC could trigger the local and systemic resistance to PXO99. Notably, no significant toxicity was observed on plants and mice after excessive foliar treatment or feeding tests, respectively. Overall, our research revealed that VMSNs are an effective, systemic, and safe nano-pesticides for controlling rice diseases. Boosting the immune responses may be associated with the transporting of nanoparticles.