Abstract
Gut bacteria play a significant role in host insect. This study evaluated detail difference of midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible and field-caught populations of diamondback moth, and studied phenomics of the predominant midgut bacterium Enterococcus mundtii. Cultivable bacteria revealed that E. mundtii and Carnobacterium maltaromaticum dominated the bacterial populations from deltamethrin-resistant and deltamethrin-susceptible larval midguts, whereas E. mundtii was predominant in field-caught population. Illumina sequencing analysis indicated that 97% of the midgut bacteria were from the phyla Firmicutes, Proteobacteria and Cyanobacteria. Both resistant and susceptible populations had more Enterococcus and Carnobacterium. Enterococcus, Carnobacterium, Bacillus, and Pseudomonas were predominant in the field-caught population. A phenomics analysis revealed that E. mundtii was able to metabolize 25.26% of the tested carbon sources, 100% of the nitrogen sources, 100% of the phosphorus sources and 97.14% of the sulfur sources, had a wide range of osmolytes and pH conditions, and showed active deaminase activity but no decarboxylase activity. This is the first report regarding different populations of DBM midgut bacteria analyzed using both high-throughput DNA sequencing and cultivation methods, and also first report concerning the phenomics of E. mundtii. The phenomics of E. mundtii provide a basis for the future study of gut bacteria functions.
Highlights
Gut bacteria play a significant role in host insect
This study investigated the larval midgut bacteria from deltamethrin-resistant, Well Substrate PM1 A02 L-Arabinose A03 N-Acetyl-D-Glucosamine A06 D-Galactose A10 D-Trehalose A11 D-Mannose B02 D-Sorbitol B03 Glycerol B08 D-Xylose B11 D-Mannitol C04 D-Ribose PM2 A03 α-Cyclodextrin A04 ß-Cyclodextrin A05 γ-Cyclodextrin A12 Pectin B01 N-Acetyl-D-Galactosamine B04 Amygdalin B05 D-Arabinose
A statistical analysis of the cultivable bacterial populations obtained on two different media did not show significant differences in the bacterial populations, indicating that the media composition did not appear to affect the cultivable bacterial strains, as previously demonstrated[27,36]
Summary
Gut bacteria play a significant role in host insect. This study evaluated detail difference of midgut bacteria in deltamethrin-resistant, deltamethrin-susceptible and field-caught populations of diamondback moth, and studied phenomics of the predominant midgut bacterium Enterococcus mundtii. A phenomics analysis revealed that E. mundtii was able to metabolize 25.26% of the tested carbon sources, 100% of the nitrogen sources, 100% of the phosphorus sources and 97.14% of the sulfur sources, had a wide range of osmolytes and pH conditions, and showed active deaminase activity but no decarboxylase activity This is the first report regarding different populations of DBM midgut bacteria analyzed using both high-throughput DNA sequencing and cultivation methods, and first report concerning the phenomics of E. mundtii. Though the current level of knowledge is too limited to indicate a relationship between gut symbioses and insecticide resistance, these suggest that adjusting the gut bacteria, replacing normal microbiota with exogentic one, associated with host insects may help manage their population in the future. Because it could allow broad phenotypic testing at a time, provide an immediate sense of the phenotypic range of a microorganism, and is much easier to perform[31], PM analysis has been widely used to analyze the phenotypes of many bacteria, such as Escherichia coli[31], Ralstonia solanacearum[32], Bacillu subtilis[33], Pseudomonas aeruginosa and Enterococcus faecalis[31]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
