Midday depression of photosynthesis and effects of mist spray in citrus

Abstract

AbstractDiurnal variations of gas exchange, chlorophyll a fluorescence and some related biochemical characteristics in sun‐acclimated mature citrus leaves of mist‐sprayed (treatment) and unsprayed (control) trees were compared on sunny days during summer to identify the environmental and physiological factors limiting carbon gain in citrus tree canopies. At midday, net photosynthesis and maximal photochemical efficiency of photosystem II (Fv/Fm) in citrus leaves decreased significantly under control conditions, but the decrease was mitigated by mist spraying. Although the content of malondialdehyde, hydrogen peroxide and activities of antioxidant enzymes increased at midday in both mist‐sprayed and control leaves, they were much higher in control leaves than in mist‐sprayed leaves. The level of D1 protein decreased significantly in control leaves at midday and then was partly recovered later, while that in treated leaves changed to a much lesser extent because of alleviation of photoinhibition by mist spraying. Both the fast and the slow phases of millisecond‐delayed light emissions in treated citrus leaves were higher than those in control leaves, indicating that mist spraying protects the normal operation of the photosynthetic apparatus in leaves. Mist spraying also reduced leaf temperatures and the ratio of air to leaf vapour pressure deficit (ALVPD), leading to increases in stomatal conductance (gs) and alleviation of photoinhibition at midday. It is concluded that the decline of leaf gs under high‐ALVPD conditions in summer is an important factor contributing to midday depression of photosynthesis in citrus, and mist spraying is effective in alleviating midday depression of photosynthesis in citrus leaves.