Micturition evoked by glutamate microinjection in the ventrolateral periaqueductal gray is mediated through Barrington’s nucleus in the rat

Abstract

Neural tracing experiments have demonstrated a direct spinal projection to Barrington’s nucleus and a possible indirect pathway to Barrington’s nucleus via the periaqueductal gray. We sought to identify the role of the periaqueductal gray matter in micturition in urethane-anesthetized rats. Blockade of micturition by focal injection of cobalt chloride was used to identify sites critical to micturition. These sites were located near the ventral margin of the caudal ventrolateral periaqueductal gray and in Barrington’s nucleus. l-Glutamate injections into caudal regions of the periaqueductal gray evoked bladder contraction with coordinated sphincter activation. Additional l-glutamate sites with a similar pattern of response and sites where sphincter activation was produced without bladder contraction were found more rostrally and dorsally in the periaqueductal gray. Activation of bladder contractions by l-glutamate injection in the ventrolateral periaqueductal gray was blocked by prior injection of cobalt chloride into Barrington’s nucleus. From these data we propose that ventrolateral periaqueductal gray is functionally important to micturition in the urethane-anesthetized rat. Further, we have shown that a periaqueductal gray to Barrington’s nucleus pathway is functionally relevant to central mediation of bladder contraction.