Mict Improves Fgf21 And Klb Expression Better Than Hiit In Obese Mice

Abstract

(No relationships reported) Background: Fibroblast growth factor 21 (FGF21), a key factor to prevent and treat overweight and obesity, regulates glucose, lipid, and energy metabolism. Besides, FGF21 needs β-Klotho (KLB) as a co-receptor to combine with the FGF receptor (FGFR) effectively and inter the target cell. However, it is unclear what condition promotes FGF21 and KLB expression in different tissue. PURPOSE: The goal of this preliminary study is to explore FGF21 and KLB expression related to two forms of exercise: moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) METHODS: Mice were randomly divided into four groups (n=8 per group): MICT, HIIT, sedentary lifestyle (SED), and control (CON). Three groups, MICT, HIIT, and SED were fed on the high-fat diet (HFD) to induce obesity and CON was fed on the standard chow (Con) for 12 weeks. Exercise was performed on a motorized treadmill for further eight weeks and the diet continued in each group. RESULTS AND DISCUSSIONS: (1)In mRNA level, MICT was more effective than HIIT in promoting FGF21 and KLB expression in liver (fgf21: 12.44±3.95 vs. 0.91±1.09, p<0.01; klb: 5.17±3.54 vs. 0.225±0.10, p<0.01), brown adipose tissue (BAT) (fgf21: 96.37±29.72 vs. 58.30±21.73, p<0.05; klb: 12.33±5.20 vs. 0.32±0.20, p<0.01), and muscle (fgf21: 10.17±5.81 vs. 5.49±9.09, p>0.05; klb: 42.83±19.07 vs. 10.33±14.50, p<0.01); (2) In protein level, MICT was more effective than HIIT in promoting FGF21 and KLB expression in liver (fgf21: 1.63±0.31 vs. 1.31±0.27, p>0.05; klb: 3.51±0.58 vs. 1.63±0.32, p<0.01), BAT (fgf21: 3.35±0.32 vs. 1.42±0.27, p<0.05; klb: 2.34±0.50 vs. 0.74±0.25, p<0.01), and muscle (fgf21: 1.32±0.12 vs. 0.72±0.12, p<0.05; klb: 2.17±0.12 vs. 1.46±0.06, p<0.01). CONCLUSION: MICT improves FGF21 and KLB expression in the liver, BAT, and muscle better than HIIT in obese mice.