Abstract
Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects.
Highlights
Silver is known as strong antimicrobial agent due to its toxicity against most microorganisms including bacteria, fungi, and viruses [1]
The obtained results were in line with the findings of other research [8,9,10]. They found that by increasing the amount of plant extract, the concentration of the bioreductants increased in the extract, which in turn increased the concentration of the formed Ag NPs
Gram-negative (E. coli) bacteria during incubation indicated that the diameter of the clear zone for synthesized Ag NPs in the plate containing S. aureus and E. coli were 16 and 10 mm, respectively (Figure 7)
Summary
Silver is known as strong antimicrobial agent due to its toxicity against most microorganisms including bacteria, fungi, and viruses [1]. Green synthesis of Ag NPs, using plants and their derivativeextracts extractsas as an alternative method the conventional and NP chemical. NP derivative an alternative method for thefor conventional physical physical and chemical fabrication fabrication methods, has attracted considerable attention in recent years [1,6]. Various methods, has attracted considerable attention in recent years [1,6]. Various metabolites existing metabolites insugars, the plants including sugars, alkaloids, phenolic acids, terpenoids, polyphenols, in the plants existing including alkaloids, phenolic acids, terpenoids, polyphenols, and proteins play an and proteins play an important roleofinsilver the bioreduction of silver ions to. Leaf of walnut contains malic acid, sucrose, α-tocopherol, 3-Oproteins, carotenoids, lipids, and alkaloids. Leaf of walnut contains malic acid, sucrose, α-tocopherol, caffeoylquinic acids, and quercetin.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
