Abstract
We describe a high sensitivity and rapid sensing technology for the detection and determination of surface-bound proteins/enzymes and potentially even DNA/RNA. Low power microwave pulses are used to “trigger” chemiluminescence “on demand” at the tips of planar triangular structures. When the triangles are deposited on 40 nm thick continuous silver films with a spacer layer, the intense pulsed chemiluminescence photon bursts become highly polarized and directional through the back of the film, as compared to traditional weak free-space isotropic emissions. Subsequently, microwave-triggered surface plasmon coupled chemiluminescence (MT-SPCC) appears to be a significant improvement over traditional chemiluminescence “slow glow” for surface assays, as well as other surface plasmon coupled fluorescence based assay technologies, such as surface plasmon coupled emission (SPCE) and surface plasmon coupled fluorescence (SPCF). Mw − Microwave pulses to locally “trigger” chemiluminescence, No Mw − No Microwave pulses, i.e. traditional chemiluminescence “slow glow”.
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