Microwave treatment for sterilization of phytoplankton culture media

Abstract

A standard microwave oven for the sterilization of phytoplankton culture media and apparatus was tested. Elimination of bacterial, algal, and fungal contaminants is achieved in < 10 min for a 1.5 1 vol. of seawater in a Teflon bottle. Empty culture tubes and vessels are sterilized in 5 min. The technique is quick, easy, reliable, and does not contaminate with trace metals or cause precipitation. A sample protocol and numerous precautions are discussed.