Abstract
A comparison is made between microwave and water-bath thawing on the survival of tissue culture cells which have been frozen to −79 °C, with DMSO and HES protective agents (freezing rate: 20 °C per min). The microwave thawing rates, selected in the range 100–300 °C/min, are comparable to those achieved with a water bath (115 °C/min). Microwave thawing is stopped at various temperatures between 0 and 40 °C, and survival, determined by cell plating indicated that the microwave thawing is acceptable. Survival rates tend to be highest when the rapid microwave thawing terminated at temperatures just above 0 °C.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
