Abstract
The enzymatic production of prebiotic xylooligosaccharides (XOS) has become an attractive way to valorise lignocellulosic biomass. However, despite numerous xylanases reported for potential use in the production of XOS, most of the family GH10 also produce xylose. This monosaccharide can negatively affect the selectivity to stimulate the growth of intestinal microorganisms beneficial to human health. In this work, thermostable alkali-tolerant xylanase (BhXyn10A) from Bacillus halodurans S7 has been used to produce XOS under conventional convective heat transfer and microwave radiation. The microwave-assisted reaction markedly decreases the xylose content in the hydrolysates and significantly increases the yield of XOS, compared to conventional heating. Molecular dynamics simulations of BhXyn10A have shown increased fluctuations of the amino acids of the aglycone subsites suggesting that these subsites can determine the production of xylose. Thus, microwave heating could affect the amino acid fluctuations in the aglycone subsites reducing the xylose formation. These findings open up new avenues in enzyme technology for the production of XOS.
Highlights
Lignocellulosic biomass has been gaining increased attention because of its potential as a raw material for the biochemical industry from a biore nery perspective
Particular interest has been put into the production of XOS, they can selectively stimulate the growth of probiotic bi dogenic and lactic acid bacteria residing in the human gut.[2]
It has been found that it is mostly XOS with a degree of polymerization (DP) from 2 to 4 that selectively induce the growth of bene cial microorganisms in the human digestive tract.[2]
Summary
Lignocellulosic biomass has been gaining increased attention because of its potential as a raw material for the biochemical industry from a biore nery perspective. Hemicelluloses can be transformed into several molecules such as xylitol, erythritol, ferulic acid, furfural, ethanol, lactic acid, and xylooligosaccharides (XOS).[1]. Particular interest has been put into the production of XOS, they can selectively stimulate the growth of probiotic bi dogenic and lactic acid bacteria residing in the human gut.[2] it has been found that it is mostly XOS with a degree of polymerization (DP) from 2 to 4 that selectively induce the growth of bene cial microorganisms in the human digestive tract.[2] the xylose (DP1) content should be minimized to enhance the quality of the prebiotic mixture
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