Abstract
The extraction and separation of docosahexaenoic acid (DHA)-rich oils, proteins, and polysaccharides from microalgae is a challenge for industrial commercialization of DHA, such as drying and dewatering, cell wall disruption, laborious conventional extraction procedures and exploitation of large volumes of solvents. To address these concerns, a novel microwave-assisted three-liquid-phase salting-out extraction (MA-TLPSOE) was used to separate DHA-rich oils, proteins, and polysaccharides from wet cultivation broths of Schizochytrium limacinum SR21. The optimized MA-TLPSOE system was composed of 30% (w/w) n-hexane/13% (w/w) ethanol/9% (w/w) sodium carbonate and treated at high microwave power (650W) for 30s. Under optimized conditions, 100±0.59% oil and 100±0.64% DHA were partitioned in the top n-hexane phase while 93.75±0.25% of polysaccharides and 92.96±0.43% of proteins were distributed in the solid-phase interface. The effects of microwave power and duration, sodium carbonate, n-hexane, ethanol concentration and extraction time were studied. When the optimized system was enlarged from 20g to 60g, the yields of all effective components were almost the same. Thus, the MA-TLPSOE system is a promising and attractive technology, which could be used for the separation and extraction of various effective ingredients from complex natural products in a single step with significant recovery yield.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.