Abstract
This work describes a new synthesis method for core–shell magnetite nanoparticles with a secondary silica shell, functionalized with a linker system (Fe3O4-PABA-SiO2-linker) using a microwave-assisted heating technique. The functionalized solid nanomaterial was used for the nanophase synthesis of peptides (Fmoc route) as a solid support. The co-precipitation method was selected to obtain magnetite nanoparticles and sol–gel technique for silica coating using a microwave-assisted (MW) procedure. The magnetic properties of the nanoparticle core offer the advantage of a quick and easy alternative for the magnetic separation of the product from the reaction mixture, facilitating all the intermediary washing and separation operations. The intermediate and final materials were analyzed by advanced characterization methods. The effectiveness of the nanophase peptide synthesis using this nanostructured material as solid support was demonstrated for a short peptide sequence.
Highlights
Core–shell nanoparticles demonstrate significant advantages compared to classic materials due to their unique properties [1], related to important applications in the field of biomedicine, such as targeted drug delivery, magnetic resonance imaging (MRI), magnetic separation and many other fields of scientific or technological interests
The present study is based on our previous work [9], related to nanophase peptide synthesis, focusing on the nanophase support synthesis optimization by an microwave irradiation (MW)-assisted process, on more accessible linker systems and on the advantage of a higher purity of the final product by the TFA cleavage of the HMBA linker-peptide system
The efficiency of the newly synthesized nanostructured system MW-NPS-2-APSHMBA was highlighted by using it as a solid support in nanophase peptide synthesis
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. The sol–gel method involves the use of an alkoxy precursor, the most widely used being TEOS as the main agent for the silica shell build-up, in the presence of a basic catalyst (ammonium hydroxide) [4]. Microwave-assisted synthesis is a fast, low-cost, and a low-energy method for nanoparticle synthesis [13], with several advantages, such as reduced reaction time, high reproducibility, aggregation control and significantly improved diffusional access. The present study is based on our previous work [9], related to nanophase peptide synthesis, focusing on the nanophase support synthesis optimization by an MW-assisted process, on more accessible linker systems and on the advantage of a higher purity of the final product by the TFA cleavage of the HMBA linker-peptide system. An improved characterization of the final peptide by FT-ICR HR-MS analysis was performed, to prove the VIK sequence
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