Abstract
AbstractChemical fixatives and conventional fixation times used for standardized preservation of tissues can result in serious alterations in morphology as a consequence of solubilization and conformational changes of proteins and lipids. These untoward changes typically result in compromised antigenicity of many tissue proteins. With the advent of microwave energy (MWE) processing, tissue antigens can be distinctly better preserved, antigen retrieval made more replicate, and histochemical and immunochemical reactions made ultrafast. The present study had two objectives: to compare MWE-processed skin with skin conventionally processed for ultrastructural integrity and to evaluate MWE presentation of antigenicities of selected skin proteins that are usually sensitive to conventional chemical processing. Skin proteins selected were bullous pemphigoid antigen (BPA), laminin and type IV collagen. Biopsied skin from haired guinea pigs was MWE-processed for routine peroxidase immunocytochemistry according to a ...
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