Abstract
Monosaccharides are usually analyzed by use of automatic monitors or enzymatic immunoassays. However, gas chromatography (GC) is an accurate and precise technique for galactose quantification (1), and it is regarded as a reference method for glucose (2). GC procedures require a long derivatization time in 2 consecutive reactions of 60–90 min to generate the aldonitrile pentaacetate derivative. Fast derivatization techniques are often requested today because the bottleneck for sample throughput has moved from analysis to sample preparation. Recently, we dramatically decreased the derivatization time for sugars (mono- and disaccharides) in GC analysis (3) by using trimethylsilyl-oxime derivatives. This derivative, however, gives …
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