Abstract
The cytoplasmic contractile elements of adult bovine lens epithelial cells growing in long-term monolayer cultures have been visualized both by conventional electron microscopy and by immunofluorescence staining with specific antibodies directed against highly purified actin and tubulin. A complex system of microfilaments and microtubules was demonstrated in the cytoplasm of these cells. Microfilaments display several configurations including fine filamentous arrays in contact with the cytoplasmic side of the plasma membrane, bundles of fibres connected particularly to junctional complexes between adjacent cells and thick bundles of stress fibres running longitudinally throughout the cell. The sensitivity of micro-filaments and microtubules to cytochalasin B and colchicine respectively was shown by immunofluorescence staining.
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