Abstract
The means of formation of the Golgi apparatus is uncertain. In beginning these studies, it seemed that an important first step was to determine the degree of dependence of the Golgi apparatus on the nucleus. This was investigated in Amoeba proteus because these cells are readily enucleated microsurgically. Normal amebae contained multiple Golgi bodies, which declined in size and number in the absence of the nucleus, indicating that the Golgi apparatus in amebae depends upon the nucleus. The influence of the nucleus on the Golgi apparatus may be mediated through nuclear RNA synthesis and cytoplasmic protein synthesis, since the ultrastructural effects of enucleation were mimicked by treating amebae with actinomycin D to inhibit RNA synthesis and, in part, by treating cells with emetine, which inhibits protein synthesis in amebae.
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More From: Proceedings, annual meeting, Electron Microscopy Society of America
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