Microstructure of a Biocatalytic Latex Coating Containing Viable Escherichia coli Cells

Abstract

Cryogenic scanning electron microscopy (cryo-SEM) was employed to visualize the microstructure of latex coatings in which viable Escherichia coli cells were entrapped for use as biocatalysts. Cryo-SEM examination of surfaces and fracture cross sections of dry and hydrated coatings cast with or without glycerol revealed two different porosities in the films: a macroporosity in which the bacterial cells reside and a microporosity made up of the interstitial voids between partially coalesced latex polymer particles. Polymer particle consolidation and coalescence in the cell-laden coatings were at an earlier stage than in cell-free latex coatings detailed in a companion paper. Coatings cast with glycerol showed a lesser degree of latex particle consolidation and coalescence than those cast without glycerol. However, the effect of glycerol was not as pronounced as in cell-free coatings. We conclude that part of the added glycerol is sequestered inside the bacterial cells and the portion remaining outside the cells retards the latex film formation process and enhances microporosity. Two commercial acrylic acid/vinyl acetate copolymer latexes were examined. Coatings made with the polydisperse latex showed less microporosity and a greater degree of particle welding than those made with the nearly monodisperse latex.