Abstract
Current techniques in acoustic microscopy have been employed to examine the structure of tissue on a microscopic basis. Acoustic micrographs of unstained specimens of mouse kidney tissue were produced at frequencies of 100 and 220 MHz, with resolutions of 20 and 9 μm, respectively. The acoustically revealed structures were studied with reference to the established microanatomy. Preliminary results show high-contrast details corresponding to the boundaries of connective tissue supporting elements of the nephron. Various regions of the kidney, specifically, the cortex and the three medullary layers, can be differentiated acoustically. The acoustic visualization of structures at these frequencies is considered to result from scattering at connective tissue interfaces, i.e., acoustic impedance discontinuities, rather than from intrinsic absorption in the microstructures of the tissue.
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