Abstract

Yogurt is a popular fermented milk product across the world. Structure, taste, and odor characteristics are created by fermenting yogurt with diverse ingredients and lactic acid bacteria (LAB), which contribute the most to the acceptance and quality of yogurt. In this study, low-fat buffalo milk yogurts (LFBY) were produced with the enrichment of 1% (w/w) whey protein concentrate (WPC) and Ca-caseinate (Ca-CN). Yogurts were analyzed based on microstructural, microbiological, organoleptical properties; volatile compounds (solid-phase microextraction method associated with gas chromatography-mass spectrometry) during cold storage for 21 days. Yogurts enriched with WPC and Ca-CN had higher total solids, total protein contents, and pH values. A total of 36 volatile components were identified in all produced yogurts. Acetic acid, butanoic acid, acetaldehyde, acetoin, 2,3-butanedione, ethanol, and 1-heptanol were found in significant amounts and mainly contributed to organoleptical properties. Interestingly, the focused volatile compounds that improve taste and odor were higher in LFBY+WPC yogurt than in plain LFBY or LFBY+Ca-CN yogurt. The Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus viable counts were higher in LFBY+WPC yogurt than plain LFBY or/and LFBY+Ca-CN. Conclusively, yogurt enriched with 1% WPC exhibited the best organoleptical properties and volatile component concentrations. The microstructure of the LFBY with WPC was less compact and dense, and regular, with tiny pores and long and individualized casein filaments than the other treatments. The microstructure of the Ca-CN samples caused a compact structure and coarse than in the control yogurt.

Highlights

  • Yogurt enriched with whey protein concentrate (WPC) was the best sample concerning the flavor, appearance, body, texture, and total acceptability

  • low-fat buffalo milk yogurts (LFBY) treatments were produced with 1% (w/w) WPC and Ca-CN enrichment

  • The data reported that WPC and Ca-CN could be used to produce LFBY with improved volatile compounds, pH, and organoleptical attributes during storage

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Summary

Introduction

Yogurt has been increasingly accepted as a fermented dairy product in the diet planning for different communities. Camel, and buffalo milks can be used individually or in combinations for yogurt production [1]. Buffalo milk (BM) has a higher fat and total solids (TS) level than cow milk [2]. Low-fat yogurt is the most popular among these dairies, but it may have flavor, aroma, and texture challenges due to its low-fat content [3]. The flavor is an important impact on quality. Many ingredients have been applied to improve the organoleptic, chemical, textural, and nutritional characteristics of yogurt and other fermented milk products such as whey protein concentrate (WPC), Ca-caseinate

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