Abstract
In this study, yellow poplar, as a raw material, was subjected to pretreatment with oxalic and sulfuric acid. We then investigated the characteristics of lignin degradation and enzyme adsorption in terms of microstructural changes, depending on the pretreatment conditions. Statistical analysis revealed that the glucan and lignin content of the sulfuric acid- and oxalic acid-pretreated biomass did not differ under the same pretreatment conditions, irrespective of the catalyst used. In contrast, difference in the degradation characteristics of lignin in the cell wall (secondary cell wall) and different cell types (vessel and wood fiber) of pretreated biomass were observed by confocal laser scanning microscopy and time of flight-secondary ion mass spectrometry (ToF-SIMS). ToF-SIMS revealed high enzyme adsorption in the middle lamella of wood fibers in the oxalic acid-pretreated biomass. In the sulfuric acid-pretreated biomass, enzyme adsorption was high in the secondary cell wall of wood fibers. G-type lignin showed a high enzyme adsorption capacity. Therefore, irreversible enzyme adsorption, particularly at the middle lamella, was higher in the oxalic acid-pretreated biomass than in the sulfuric acid-pretreated biomass.
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