Abstract
The extinctions (optical densities) of cells incubated for acid phosphatase in a histochemical azo-coupling procedure (naphthol AS-BI prosphate-hexazotized pararosaniline) have been measured microspectrophotometrically as a function of the incubation time and substrate concentration. A microcuvette was designed for the incubation. The amount of the reaction product in the cells at 23±1°C was found to be proportional to the incubation time for at least 40 min. Lineweaver-Burk plots were linear for some cells while in others nonlinear plots suggested the presence of two or more enzymes. This suggestion was supported by results obtained from polyacrylamide gel electrophoresis experiments.
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