Abstract

1. 1. The amino acid incorporation system of the combined microsomalsoluble fraction of livers from rats pretreated with DMNA in vivo, was impaired to a marked extent. 2. 2. Preincubation of normal rat liver slices with DMNA decreased the incorporation of amino acid into the proteins. The effect was dependent upon oxygen. 3. 3. The amino acid incorporation into the proteins of rat hepatoma slices was not impaired under similar conditions. 4. 4. The microsomes of normal rat liver but not those of rat hepatoma contained an active N-demethylation enzyme which produced formaldehyde from DMNA. Rat liver mitochondria were inactive in this respect. 5. 5. It is concluded that the formaldehyde or the initially-formed methylol derivative of DMNA reacts with susceptible groups in the microsomes and thus blocks the amino acid incorporation system. 6. 6. The effect of DMNA was rather specific in that respiration, glycolysis and a number of other enzymic activities were not impaired under the present experimental conditions.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.