Microscopy-based morphological characterization of rugose spiraling whitefly, (Aleurodicus rugioperculatus Martin)-an exotic pest on coconut in India.

Abstract

Precise identification of exotic whiteflies is a prerequisite to curb the invasive potential on to a new geographical location and to evolve effective management strategies. Conventionally, whitefly taxonomy is based on the description of the fourth-instar nymph or puparium, however, in the current investigation egg to adult morphology along with morphometrics of rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus Martin was critically analyzed and various morphological characteristics were illustrated. The morphometric analysis revealed that in the immature life stages of the pest, the length: width ratio decreased with each successive life stage, such as the egg, first, second, third, and fourth instar (2.472 ± 0.071, 1.913 ± 0.020, 1.550 ± 0.045, 1.297 ± 0.034, and 1.174 ± 0.058 mm), respectively. Across different nymphal instars, the shape of lingula was greatly modified from tongue-like to triangle-shaped. The number and distribution of compound pores also vary among the different nymphal stages. The ultra-structures of the antenna through electron-microscopy depicted finer details of sensory cones. The modulation in the shape and the structural arrangement of microtrichia on the plate was illustrated. The study indicated accurate diagnosis of various stages of RSW for effective interception of goods at quarantine stations and thus preventing the entry of exotic pests into the country. RESEARCH HIGHLIGHTS: Microscopy-based (light and scanning electron microscopy) morphological characterization of rugose spiraling whitefly, (Aleurodicus rugioperculatus Martin).