Microscopical observations on the regenerating tail of tsinling dwarf skink (Scincella tsinlingensis)

Abstract

Although the key steps of tail regeneration in lizards are well understood, further investigations involving skinks can provide the field of regeneration research with additional information. In order to characterize the cytoarchitecture of tail regeneration in Scincella tsinlingensis, an endemic species in China, its histological events and growth trends are investigated. The rate of tail regeneration varies with the season: it proceeds faster in summer and autumn than it does in winter and spring. Tail regeneration of S. tsinlingensis is summarized as wound healing, blastema formation, cell differentiation and tail growth, which can be subdivided into seven stages. Wound healing following tail loss, begins with an obvious outgrowth undergoing re-epithelialization. Numerous proliferating mesenchymal-like cells aggregate near the distal end of the severed spinal cord to form the blastema. The expanding blastema is invaded by blood vessels, nerves and ependyma. A cartilaginous skeleton is formed around the ependymal tube and the muscle starts to differentiate. The keratinization of epidermis coincides with scale formation. Pigmentation eventually occurs in the regenerated tail. Tail regeneration in S. tsinlingensis is an epimorphic kind of regeneration that is also known as blastema-mediated. Structure and composition of the regenerated tail, including its cytoarchitecture, represent a conserved pattern of regeneration also known from other lizards.