Microscopical and functional aspects of calcium-transport and deposition in terrestrial isopods

Abstract

Terrestrial isopods (Crustacea) are excellent model organisms to study epithelial calcium-transport and the regulation of biomineralization processes. They molt frequently and resorb cuticular CaCO 3 before the molt to prevent excessive loss of Ca 2+ ions when the old cuticle is shed. The resorbed mineral is stored in CaCO 3 deposits within the ecdysial gap of the first four anterior sternites. After the molt, the deposits are quickly resorbed to mineralise the posterior part of the new cuticle. The deposits contain numerous small spherules composed of an organic matrix and amorphous CaCO 3, which has a high solubility and, therefore, facilitates quick mobilization of Ca 2+ and HCO 3 - ions. During the formation and resorption of the deposits large amounts of Ca 2+, HCO 3 - and H + are transported across the anterior sternal epithelial cells. Within the last years, various light and electron microscopical techniques have been used to characterize the CaCO 3 deposits and the cellular mechanisms involved in biomineralization. The work on the CaCO 3 deposits includes studies on the ultrastructure of the deposits, the sequence of events during deposit formation and dissolution, and the mineral composition of the sternal deposits. The differentiation of the anterior sternal epithelial cells and the mechanisms of epithelial ion transport required for the mineralization and demineralisation of the deposits was studied using various analytical light and electron microscopical techniques including polarized light microscopy, immunocytochemistry, electron microprobe analysis, electron energy loss spectroscopy and electron spectroscopic imaging. Comparative analysis of deposit morphology and the differentiation of the sternal epithelia provide information on the evolution of CaCO 3 deposit formation in relation to the degree of adaptation to terrestrial environments.