Abstract

A novel nonlinear confocal microscopic imaging system based on Raman induced Kerr effect spectroscopy (RIKES) is presented in this paper. The three-dimensional (3-D) microscopic imaging theory is derived with the Fourier imaging theory and nonlinear optical principle. The impact of RIKES on the spatial resolution and imaging properties of confocal microscopic imaging system has been analyzed in detail by the imaging theory. It’s proved that the RIKES nonlinear microscopic imaging system can effectively improve the imaging contrast and provide more characteristic information on Raman spectrum and optical nonlinear Kerr effect, thus greatly improving the imaging quality of confocal microscopic imaging system. It’s shown that the spatial resolution of RIKES confocal microscopic imaging system is higher than that of two-photon confocal microscopic imaging system.

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