Abstract
Cymbidium sp. is one of the most popular orchid desired in public, it means high productivity is needed in its fulfillment. One of the obstacle of Cymbidium sp. orchid in order to fullfilment of productivities are the stress and orchid’s seed size which is very small. Vesicular Arbuscular Mycorrhizae is a symbiont fungus that can help absorption of nutrients in stress phase. VAM help Cymbidium sp. to maintenance and increase the productivity. This research aimed to determined VAM species and colonization in rhizospher of Cymbidium sp. orchid. This research has been conducted from November 2020 untill April 2021. Spore isolation was conducted by using wet shievings method, meanwhile VAM colonization was conducted by using roots staining techniques. The result showed there were 4 genus consisted by 6 different type of spores which has been found. There was Glomus (2 types), Dentiscutata (2 types), Acaulospora (1 type), and Gigaspora (1 type). Colonization of VAM in sample plant has been known by presence of VAM special structure, there were vesicular and internal hyphae.
Highlights
Orchid (Orchidaceae) are plants that have high diversity and are popular among the public
The result of spore characterization which has been isolated from the rhizosphere of Cymbidium sp. obtained four genera with six different types of Versicular Arbuscular Mycorrhizae (VAM)
The result of spore characterization was dominated by Glomus, Dentiscutata, and followed by Acaulospora and Gigaspora
Summary
Orchid (Orchidaceae) are plants that have high diversity and are popular among the public. Based on data from Badan Pusat Statistik (“Outlook Komoditas Pertanian Subsektor Hortikultura Anggrek,” 2015) orchid production have inclined to ±16,53 million unit per year. This fact is evidence that market demand for orchid is directly proportional to fulfillment. It is important to maintain the stability and maximality of orchid production. One of the obstacle factors to maintain and maximize orchid production is at germination process, orchid’s seed does not have endosperm which makes natural germination mechanism hampered. According to Andersen and Rasmussen (1995), the existence of mycelium mycorrhizae that are compatible can help the process of initiation and inclined orchid germination significantly. Versicular Arbuscular Mycorrhizae (VAM) have a role in organic and inorganic nutrition such as phosphor, carbon, water, and nitrogen provision can increase the efficiency of groundwater usage by plants so that it can help to increase the vegetative growth rate of orchid plant (Miransari, 2014)
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